Harbin Medical University realizes efficient cultivation of neural stem cells

Release date: 2007-12-14

Harbin Medical University realizes high-efficiency training of neural stem cells. The first clinical college of Harbin Medical University researchers use small neurosphere passage method to culture neural stem cells in vitro, which can be passaged for 25 generations in 10 months, from single cells to 100,000 cells. . This method has been widely applied in the key laboratory of cell transplantation of the Ministry of Health of Harbin Medical University, and has published many academic papers.
Stem cell research is undoubtedly the hottest research field in 2007, and researchers from home and abroad have also made great achievements in stem cell basic research and applied research. Clinically, axonal regeneration and functional recovery after spinal cord injury are still insurmountable obstacles, and the destructiveness of spinal cord injury is severe and long-lasting for the injured. At present, many scholars have a strong interest in the development of stem cell culture and application potential, because these cells can provide neurons, neuronal cells as a target for regenerating axons, and can promote the reconstruction of neural circuits.
Dr. Liang Peng, Department of Neurosurgery, Harbin Medical University, a long-term in vitro culture system of neural stem cells established by small neurosphere passage method. It was confirmed that in the cell passage and induced differentiation, the neurospheres formed by stem cell proliferation were digested into small 10-20 cells. The small neurosphere can significantly improve the in vitro viability and proliferation ability of the cells. Neural stem cells can be cultured in vitro for 10 months using a small neurosphere passage method, passaged for 25 generations, and can be expanded from a single cell to 100,000 cells. After several passages, cell cryopreservation had no significant effect on cell viability, and multi-directional differentiation potential showed stability. The growth curve shows that the number of cells has been exponentially expanded after many passages, providing a large number of cell sources for the study and transplantation of neural stem cells.
On this basis, the research team also successfully established a multi-point transplantation technique for nerve stem cells with small neurospheres and spinal cords. The neurospheres were transplanted to the transverse ends of the spinal cord of rats to observe the recovery of paraplegia. The study found that the neurons induced by spinal cord transplantation in vitro and injured rat spinal cord were significantly higher than the traditional neural stem cell suspension transplantation, and the cell survival rate was significantly increased. Neuronal tracer technique was used to confirm the regeneration of the spinal cord of the injured rat spinal cord and establish a synaptic connection with the distal neurons. Consistent with this, the hindlimb motor function of paraplegic rats after spinal cord injury was also significantly improved. The research team further clarified the mechanism of neural stem cells repairing spinal cord injury. These mechanisms include: promoting the regeneration and re-myelination of injured axons, and the reconstruction of the neural circuit after spinal cord injury; at home and abroad, it is first proposed that human embryonic neural stem cells have different repair mechanisms for spinal cord injury than previous studies. In addition to the cell replacement effect, neural stem cells can promote the recanalization of the neural circuit through nutritional support, that is, the role of the cell matrix.
Spinal cord injuries often result in severe disability. There are tens of millions of patients with spinal cord injuries in the world, and there are more than one million in China. According to statistics, more than 10,000 injured and sick people are added each year in the United States. The incidence of spinal cord injury in Beijing alone is about 800 people per year, which has become one of the high-risk injuries. ——China Medicine 123 Network

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